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gRAD-MWB
For use with Mouse antibody derived detection conjugates
to analyse Whole Blood sample.
Summary:
In most applications, the specific test reagent contains both a biotinylated capture reagent and a detection conjugate. When the test reagent is
mixed with the sample, both the capture reagent and detection conjugate react with the analyte present in the sample to form a complex. The sample
mixture is applied to the round well of the gRAD, blood cells or other particles are filtered out and the liquid flows through the device,
passing the test line. The test line contains a biotin-binding protein that captures both complexed and free biotinylated capture reagent,
the ratio of the first to the second relating to the amount of analyte present in the sample. Sufficient capture reagent is present in the test
reagent to saturate the test line after running for 5-10 minutes. On saturation, the amount of analyte complex immobilized at the test line
depends on its ratio to the free capture reagent, not on the volume of sample that has crossed the test line.
The round and square application wells
Apart from the round well, the gRAD has an additional square well which can be used when the detection conjugate is inhibited by other components
present in the sample (e.g. immune response assays in which the detection conjugate binds all antibodies or a class of antibodies). In these cases
the sample is mixed with the biotinylated capture reagent before being applied to the square well, whereupon the detection conjugate is added to
the round well. The detection conjugate solution pushes the sample mixture in front of it through the gRAD to the test line, where the biotin
binding protein captures the biotinylated capture reagent. The rest of the sample mixture containing the inhibiting components continues through
the gRAD, leaving the capture reagent-analyte complex at the test line ready to react with the detection conjugate that passes over it.
Control line - for mouse antibody-derived detection conjugates
The gRAD-MWB is designed for use with mouse antibody-derived detection conjugates in that the control line contains antibodies that bind the mouse
antibody which is responsible for immobilizing the detection conjugate at the test line. A visibly coloured control line indicates that the
sample mixture or sample mixture + detection conjugate has passed correctly through the device.
Filter for whole blood or particulate samples
The gRAD-MWB is designed with a filter to permit the analysis of whole blood and samples containing particulate matter. The gold colloid conjugate
passes through the filter with the rest of the sample mixture to interact with the test and control lines.
Supply
The gRAD-MWB is supplied in a plain white box of 40 units marked only with the batch number. All other labelling is present on a plastic film
wrapping that is easily removed, allowing you to put on your own labels as required. Each unit is packed in a white foil pouch which is also
marked only with a batch number to permit your own labelling. The batch number is printed directly on the gRAD, as well as an broad arrow
indicating the direction in which the gRAD should be placed in reader. Sample identification can be written within the arrow.
Specifications:
| Dimensions: |
78 mm (L) x 20 mm (W) x 5mm (D) |
| Sample pad: |
Whole blood filter |
Sample type: |
Whole blood, serum, plasma, urine, and other aquious solutions |
| Test line: |
Biotin specific |
| Control Line: |
Mouse Ig specific |
| Detection: |
Only for use with gold colloid based detection conjugates. |
| Application volume: |
100 - 200 µl |
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